Guy Town

Nick Kroll

Nick Birch / Maury the Hormone Monster / Coach Steve / Lola Skumpy / Rick the Hormone Monster (voice)

John Mulaney

Andrew Glouberman (voice)

Jessi Klein

Jessi Glaser (voice)

Jenny Slate

Missy Foreman-Greenwald (voice)

Jason Mantzoukas

Jay Bilzerian (voice)

Maya Rudolph

Diane Birch / Connie the Hormone Monstress (voice)

Fred Armisen

Elliot Birch (voice)

Jordan Peele

Ghost of Duke Ellington (voice)

Andrew Rannells

Matthew (voice)

Harvey Fierstein

Older Guy Town Resident (voice)

John Gemberling

Tyler the Hormone Monster / Dot #1 (voice)

Ashley London

Skank (voice)

Jerry Minor

Man #3 / Singing Fish (voice)

Gil Ozeri

Man #1 / Dot #2 (voice)

Mark Rivers

Man #2 / Penis Worms (voice)

Joe Wengert

Caleb / Dot #3 / Leore Paulblart (voice)

David Thewlis

Shame Wizard (voice)

Gina Rodriguez

Gina Alvarez (voice)

Seth Morris

Greg Glaser (voice)

Richard Kind

Marty Glouberman (voice)