Ejaculation

Nick Kroll

Nick Birch / Maury the Hormone Monster / Coach Steve (voice)

John Mulaney

Andrew Glouberman (voice)

Jessi Klein

Jessi Glaser (voice)

Jenny Slate

Missy Foreman-Greenwald (voice)

Jason Mantzoukas

Jay Bilzerian (voice)

Maya Rudolph

Diane Birch / Constance the Hormone Monstress (voice)

Fred Armisen

Elliot Birch (voice)

Jordan Peele

Ghost of Duke Ellington (voice)

Andrew Rannells

Matthew (voice)

Jessica Chaffin

Shannon Glaser (voice)

Joe Wengert

Teacher (voice)

Seth Morris

Ninth Grader (voice)

Emily Altman

Olivia / Filmstrip Narrator (voice)

Jak Knight

DeVon (voice)

June Diane Raphael

Devin (voice)

Jon Daly

Judd Birch (voice)

Kat Dennings

Leah Birch (voice)

Paula Pell

Barbara Glouberman (voice)

Neil Casey

Lars (voice)

Richard Kind

Marty Glouberman (voice)