Steve the Virgin

Nick Kroll

Nick Birch / Maury the Hormone Monster / Coach Steve / Lola Skumpy / Rick the Hormone Monster (voice)

John Mulaney

Andrew Glouberman (voice)

Jessi Klein

Jessi Glaser (voice)

Jenny Slate

Missy Foreman-Greenwald (voice)

Jason Mantzoukas

Jay Bilzerian (voice)

Maya Rudolph

Diane Birch / Connie the Hormone Monstress (voice)

Fred Armisen

Elliot Birch (voice)

Jordan Peele

Ghost of Duke Ellington (voice)

Andrew Rannells

Matthew (voice)

Mark Rivers

Vendor (voice)

Craig Robinson

End Credits Singer (voice)

Joe Wengert

Caleb (voice)

David Thewlis

Shame Wizard (voice)

Jak Knight

DeVon (voice)

Heather Lawless

Jenna Bilzerian (voice)

June Diane Raphael

Devin (voice)

Gina Rodriguez

Gina Alvarez (voice)

Jack McBrayer

Pubic Hair #1 (voice)

Seth Morris

Greg Glaser (voice)

Neil Casey

Lars (voice)