American Gladiators 'un 2 sezonu 'da samedi 1 septembre 1990 'dan samedi 23 février 1991'a kadar yayınlandı. 26 bölümlerinden oluşuyor, ilkinin adı "Round One Preliminaries" ve sonuncusunun adı "Alumni Show".
In this challenging game show, four contestants were pitted each week against the four athletically trained gladiators, two female and two male. Their strength, agility and endurance were tested in various, constantly changing contests against the powerful gladiators, with the highest scorers advancing to the exciting elimination tournament.