Sword of Shikata

Neil Patrick Harris

Peter Parker / Spider-Man (voice)

Lisa Loeb

Mary Jane Watson (voice)

Ian Ziering

Harry Osborn (voice)

Angelle Brooks

Indira "Indy" Daimonji (voice)

SuChin Pak

Alison Tomita (voice)

Keith Carradine

J. Jonah Jameson

Michael Dorn

Kraven the Hunter

Virginia Madsen

Silver Sable

Rob Zombie

Dr. Curt Connors / the Lizard

Michael Clarke Duncan

Wilson Fisk / the Kingpin

Cree Summer

Professor Williams / Jolenna

Stan Lee

Frank Elson

Ed Asner

Officer Barr (voice)

Clancy Brown

Raymond (voice)

Gina Gershon

Shikata (voice)

John C. McGinley

Richard Damien (voice)

Eve

Cheyenne / Talon (voice)

Keith David

Agent Mosely (voice)

Jennifer Hale

Female Reporter (voice)