Duke

Nick Kroll

Nick Birch / Maury the Hormone Monster / Coach Steve / Lola Skumpy / Rick the Hormone Monster (voice)

John Mulaney

Andrew Glouberman (voice)

Jessi Klein

Jessi Glaser (voice)

Jenny Slate

Missy Foreman-Greenwald (voice)

Jason Mantzoukas

Jay Bilzerian (voice)

Maya Rudolph

Diane Birch / Connie the Hormone Monstress (voice)

Fred Armisen

Elliot Birch (voice)

Jordan Peele

Ghost of Duke Ellington (voice)

Quinta Brunson

Girl (voice)

Jak Knight

Tall Guy (voice)

Seth Morris

(voice)

Tawny Newsome

(voice)

Chris Redd

(voice)

Retta

Duke's Mother (voice)

Craig Robinson

Harvey Brooks (voice)

Wanda Sykes

Ghost of Harriet Tubman (voice)

Jaboukie Young-White

(voice)

Gil Ozeri

(voice)

Mark Rivers

(voice)