Spider-Man Dis-Sabled

Neil Patrick Harris

Peter Parker / Spider-Man (voice)

Lisa Loeb

Mary Jane Watson (voice)

Ian Ziering

Harry Osborn (voice)

Angelle Brooks

Indira "Indy" Daimonji (voice)

SuChin Pak

Alison Tomita (voice)

Keith Carradine

J. Jonah Jameson

Michael Dorn

Kraven the Hunter

Rob Zombie

Dr. Curt Connors / the Lizard

Michael Clarke Duncan

Wilson Fisk / the Kingpin

John C. McGinley

Richard Damien

Gina Gershon

Shikata

Cree Summer

Professor Williams / Jolenna

Jennifer Hale

Female Reporter / Female News Announcer

Stan Lee

Frank Elson

Ed Asner

Officer Barr (voice)

Virginia Madsen

Silver Sable (voice)

Clancy Brown

Policeman (voice)

Jane Lynch

Oscorp Executive (voice)

Karen Maruyama

Head Protestor (voice)

Rino Romano

Muang / Drip Den guy (voice)