Duke

Nick Kroll

Nick Birch / Maury the Hormone Monster / Coach Steve (voice)

John Mulaney

Andrew Glouberman (voice)

Jessi Klein

Jessi Glaser (voice)

Jenny Slate

Missy Foreman-Greenwald (voice)

Jason Mantzoukas

Jay Bilzerian (voice)

Andrew Rannells

Matthew MacDell (voice)

Maya Rudolph

Diane Birch / Constance the Hormone Monstress (voice)

Fred Armisen

Elliot Birch (voice)

Jordan Peele

Ghost of Duke Ellington (voice)

Jessica Chaffin

Shannon Glaser (voice)

Quinta Brunson

Girl (voice)

Jak Knight

Tall Guy (voice)

Seth Morris

(voice)

Tawny Newsome

(voice)

Chris Redd

(voice)

Retta

Duke's Mother (voice)

Craig Robinson

(voice)

Wanda Sykes

Ghost of Harriet Tubman (voice)

Jaboukie Young-White

(voice)

Gil Ozeri

(voice)

Mark Rivers

(voice)